{"id":8726,"date":"2024-10-31T13:11:13","date_gmt":"2024-10-31T12:11:13","guid":{"rendered":"https:\/\/satdi.umh.es\/?page_id=8726"},"modified":"2026-01-29T20:44:37","modified_gmt":"2026-01-29T19:44:37","slug":"satdi-ic-microscopio-invertido-fret-axiovert-200-zeiss","status":"publish","type":"page","link":"https:\/\/satdi.umh.es\/en\/servicios-prestados\/instrumentacioncientifica\/servicios_ofertados\/servicios_ofertados-satdi-ic-microscopia-optica-avanzada\/satdi-ic-microscopio-invertido-fret-axiovert-200-zeiss\/","title":{"rendered":"SATDI-IC: FRET Inverted Microscope (Axiovert 200, Zeiss)"},"content":{"rendered":"

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Equipment<\/span><\/span><\/strong><\/p>\n<\/div>\n

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FRET inverted microscope<\/span><\/span>
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Axiovert 200 (Zeiss)<\/span><\/span><\/strong><\/p>\n<\/td>\n

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Elche Campus<\/span><\/span><\/strong><\/span><\/p>\n<\/td>\n

\"SATDI-IC<\/td>\n<\/tr>\n
\"Image<\/td>\n\n

This inverted microscope is optimized for both transmitted light and fluorescence imaging, as well as for performing FRET (Fluorescence Resonance Energy Transfer) assays. It features standard filters (DAPI\/FITC\/TRITC), a fast-wavelength-switching monochromator as the excitation system (OptoScan, Cairn Research), and a high-sensitivity, high-speed camera (ORCA FLASH 4.0, Hamamatsu). For FRET assays, the microscope is further equipped with the W-View Gemini dual-emission system (Hamamatsu) and a set of CFP\/YFP filters, enabling the capture of two images at different wavelengths with a single camera. The installed software allows for image acquisition and processing (HCImage, Hamamatsu).<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

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\u00a0<\/strong><\/p>\n<\/td>\n<\/tr>\n

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Applications of this system include:<\/span><\/span><\/p>\n

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    Acquisition of transmitted light and\/or fluorescence images in histology and cytology samples.<\/span><\/span><\/p>\n<\/li>\n

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    Real-time acquisition of fluorescence images for the measurement of multiple biological parameters (intracellular calcium, pH, oxidative stress, etc.) using fluorescent probes in cells.<\/span><\/span><\/p>\n<\/li>\n

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    Measurement of molecular interactions, receptor-ligand, protein-protein, protein-membrane lipids.<\/span><\/span><\/p>\n<\/li>\n

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    Studies of dynamics, conformational changes, and protein formation.<\/span><\/span><\/p>\n<\/li>\n

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    Analysis of molecular interaction distances and supramolecular dynamics.<\/span><\/span><\/p>\n<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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    Contact<\/span><\/span><\/strong><\/p>\n<\/div>\n

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    Elche Campus<\/span><\/span><\/strong><\/p>\n

    TORREGAIT\u00c1N Building <\/span><\/span>
    \n(GIS Code: E10P1049)<\/span><\/span><\/p>\n<\/td>\n

    		View location<\/span><\/span>\"Location<\/a><\/td>\n<\/td>\n\n
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    • Quesada Moll, Iv\u00e1n<\/span><\/span><\/strong><\/li>\n
    • +34 965 22 2003<\/span><\/span><\/li>\n
    • ivanq@umh.es<\/span><\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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      Current rates (2026)<\/span><\/span><\/strong><\/p>\n<\/div>\n

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      Reference<\/span><\/span><\/strong><\/p>\n<\/td>\n

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      Description<\/span><\/span><\/strong><\/p>\n<\/td>\n

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      Unit <\/span><\/span>
      \nof <\/span><\/span>
      \nmeasurement<\/span><\/span><\/strong><\/p>\n<\/td>\n

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      Unit price UMH (\u20ac)<\/span><\/span><\/strong><\/p>\n<\/td>\n

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      Unit price for Public Entities (\u20ac)<\/span><\/span><\/strong><\/p>\n<\/td>\n

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      Unit price for private entities (\u20ac)<\/span><\/span><\/strong><\/p>\n<\/td>\n<\/tr>\n

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      T-ELTG-03<\/span><\/span><\/p>\n<\/td>\n

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      Using the Zeiss Axiovert 200 FRET Microscope<\/span><\/span><\/p>\n<\/td>\n

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      hour<\/span><\/span><\/p>\n<\/td>\n

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      17.11<\/span><\/span><\/p>\n<\/td>\n

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      19.80<\/span><\/span><\/p>\n<\/td>\n

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      20.98<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

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      Observations<\/span><\/span><\/strong><\/p>\n<\/div>\n

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      The equipment has been acquired thanks to an action co-financed by the European Union through the <\/span><\/span>Operational Programme of the European Regional Development Fund<\/span><\/span><\/a> (ERDF) of the <\/span><\/span>Valencian Community 2014-2020<\/span><\/span><\/a> (PROJECT: IDIFEDER2020\/022).<\/span><\/span><\/p>\n<\/td>\n

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      Equipment<\/p>\n

      FRET inverted microscope<\/p>\n

      Axiovert 200 (Zeiss)<\/p>\n

      Elche Campus<\/p>\n

      This inverted microscope is optimized for both transmitted light and fluorescence imaging, as well as for performing FRET (Fluorescence Resonance Energy Transfer) assays. It features standard filters (DAPI\/FITC\/TRITC), a fast-wavelength-switching monochromator as the excitation system (OptoScan, Cairn Research), and a high-sensitivity, high-speed camera (ORCA FLASH 4.0, Hamamatsu). For FRET assays, […]<\/p>\n","protected":false},"author":5414,"featured_media":0,"parent":349,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_links_to":"","_links_to_target":""},"_links":{"self":[{"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/pages\/8726"}],"collection":[{"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/users\/5414"}],"replies":[{"embeddable":true,"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/comments?post=8726"}],"version-history":[{"count":0,"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/pages\/8726\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/pages\/349"}],"wp:attachment":[{"href":"https:\/\/satdi.umh.es\/en\/wp-json\/wp\/v2\/media?parent=8726"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}