FRET inverted microscope

Axiovert 200 (Zeiss)

Elche Campus

This inverted microscope is optimized for both transmitted light and fluorescence imaging, as well as for performing FRET (Fluorescence Resonance Energy Transfer) assays. It features standard filters (DAPI/FITC/TRITC), a fast-wavelength-switching monochromator as the excitation system (OptoScan, Cairn Research), and a high-sensitivity, high-speed camera (ORCA FLASH 4.0, Hamamatsu). For FRET assays, the microscope is further equipped with the W-View Gemini dual-emission system (Hamamatsu) and a set of CFP/YFP filters, enabling the capture of two images at different wavelengths with a single camera. The installed software allows for image acquisition and processing (HCImage, Hamamatsu).

Applications of this system include:

• Acquisition of transmitted light and/or fluorescence images in histology and cytology samples.

• Real-time acquisition of fluorescence images for the measurement of multiple biological parameters (intracellular calcium, pH, oxidative stress, etc.) using fluorescent probes in cells.

• Measurement of molecular interactions, receptor-ligand, protein-protein, protein-membrane lipids.

• Studies of dynamics, conformational changes, and protein formation.

• Analysis of molecular interaction distances and supramolecular dynamics.

Elche Campus

TORREGAITÁN Building
(GIS Code: E10P1049)

• Quesada Moll, Iván
• +34 965 22 2003

The equipment has been acquired thanks to an action co-financed by the European Union through the Operational Programme of the European Regional Development Fund (ERDF) of the Valencian Community 2014-2020 (PROJECT: IDIFEDER2020/022).