Super-resolution confocal inverted optical microscope

LSM900-Airyscan 2 (Zeiss)

Elche Campus

The LSM900 inverted confocal optical microscope with Airyscan 2 offers excellent performance for acquiring high-quality, high-resolution images. It allows operation in confocal or super-resolution modes.

Some features of the equipment are detailed below:

• Solid-state diode laser excitation lines: 405 nm, 488 nm, 561 nm and 640 nm

• Colibri 5 LED fluorescent lighting system with RGB-UV modules

• Objectives:
  EC Plan-Neofluar 10x/0.3
  Plan-Apochromat 20x/0.8
  LD Plan-Neofluar 20x/0.4 Corr Ph2
  Plan-Apochromat 40x/1.3 Oil DIC (UV) VIS-IR
  Plan-Apochromat 63x/1.4 Oil DIC

• Available filters:
  90 HE LED including 425/30 (DAPI, Alexa 405, Hoechst and similar), 541/30 (GFP, Fluo 4, FITC and similar), 592/25 (Cy3, TRITC, DsRed and similar) and 709/100 (Cy5, Alexa 631, TOTO-3 and similar)

• Detectors:
  It has two GaAsP detectors.
  The Airyscan 2 super-resolution detector has 32 concentrically grouped detection elements, increasing photon arrival and efficiency, increasing the signal-to-noise ratio between 4 and 8 times, and allowing a super-resolution of 120 nm.

• High-resolution motorized XY stage and motorized Z-axis focusing with 10nm steps. Several universal mounting frames.

• ZEN pro software with several associated measurement modules such as TimeLapse, Z-stack, Tiles, Shuttle&Find or Autofocus

• The Airyscan 2’s Multiplex measurement mode allows you to obtain up to four super-resolution image pixels in a single sweep, thus significantly increasing acquisition speed.

Elche Campus

TORREGAITÁN Building
(GIS Code: E10P1010)

• Serrano García, José Manuel
• +34 966 65 8448 | +34 648891391 | 51391

• Rodríguez Cañas, Enrique
• +34 965 22 2237

The equipment has been acquired thanks to an action co-financed by the European Union through the Operational Programme of the European Regional Development Fund (ERDF) and the Ministry of Science and Innovation (State Research Agency) (PROJECT: EQC2019-005842P).

Both this confocal microscope and the scanning electron microscope at IDiBE can share the same type of sample holders and use the same software, so correlative CLEM (Correlative Light-Electron Microscopy) can be easily carried out by superimposing and merging the information obtained by both techniques in the same area of ​​the sample